国标法对乳碱性磷酸酶的检测及其在巴氏消毒上的研究

采用国标法对乳中碱性磷酸酶（ALP）检测，结果显示：全乳和脱脂乳的酶活力分别为403.345、148.8147 μg/ml，变异系数分别为1.30%、2.99%。与国标法的不同之处：乳样最佳稀释比为1∶25，苯酚的最佳浓度为50 μg/ml；然后对其模拟巴氏消毒得出：63℃ 30 min基本使ALP灭活。     

杨树品比试验的天牛危害状况差异分析

本文以湖北省潜江市林科所营建的以抗天牛品系选育为主要目的的杨树对比试验林3a生的调查数据为基础,记载每木树高、胸高直径和树皮形状指数和受害状况指数等形质指标,分别空间和品系间的天牛危害程度是否存在差异进行方差分析。同时尝试运用“日本林木育种中心”开发的LSAB软件和选择指数法,分别“树高、胸径、树皮形状、被害状况”等联合形状指标对生长较好,抗性较强的无性系进行了初步选育。     

马尾松种子园营建初报

于宜昌市林科所营建马尾松种子园20hm~2。其中无性系园(马尾松一代重建嫁接种子园)13.333 hm~2,共收集154个经过子代鉴定、遗传增益大于10%的优良无性系;实生苗种子园6.667 hm~2,于枝城市、远安县、咸丰县等马尾松优良种源区选择优树145株,选用了120个优树家系苗。建园中采用了圃地小砧嫩枝诱根嫁接、一次性定植、计算机软件(SODP)优化排列设计等先进技术。     

抚仙湖流域区磷矿开采废弃地植物群落演替的研究

通过对云南省抚仙湖流域区1—5年的磷矿开采废弃地植物群落物种组成、结构及其与环境的关系等设样调查，探讨了植被恢复中植物群落演替的一般规律。调查研究表明，1—5年磷矿开采废弃地植物群落的次生演替以菊科的牛尾蒿、加蓬和1年生禾草为先锋植物迁移定居开始，而后是多年生草本和蝶形花科的波叶山蚂蝗等陆续出现并在一定地段形成优势。演替的方向符合复生演替规律；演替速度较快，表明该地区具有较强的植被恢复能力。     

Rock phosphate and vesicular-arbuscular mycorrhiza effects on growth and nutrient uptake of Faidherbia albida (Del.) seedlings in an alkaline sandy soil

Response of Faidherbia albida (Del.) to five levels of Kodjari rock phosphate (KRP) application (0, 310, 620, 1240 and 2480 ppm P, equivalent to 0, 775, 1550, 3100 and 6200 kg P/ha) and vesicular-arbuscular mycorrhizal fungi (VAMF) Glomus manihotis Howeler, Sieverding & Schenck or Glomus aggregatum Schenck & Smith emend. Koske was evaluated in an alkaline sandy soil. The F. albida seedlings grew poorly without mycorrhizal colonization and without KRP applications. For non-VAM Faidherbia, the maximum growth response and both P and N uptake in shoots was achieved with the 620 ppm P. However, even without KRP application, VAM plants achieved better results in terms of biomass. VAM plants with G. manihotis and G. aggregatum improved plant growth and increased nutrient contents at any KRP application rate. Although mycorrhizal colonization was comparable at all levels of KRP application, the impact of nutrient content of the shoot varied. Finally, VAM plants did not accumulate more biomass than non-VAM plants at 620 ppm P and above. Growth response and mycorrhizal dependency decreased as KRP applied levels increased. These results suggest that VAM Faidherbia seedlings take up more P from soil and KRP than non-VAM.     

东祁连山7种禾草根际溶磷菌筛选及其溶磷特性

采用PKO无机磷和蒙金娜有机磷选择性培养基,从东祁连山(甘肃省天祝县及周边地区)天然草地上的7种禾草根际分离出溶解无机磷菌株109株,溶解有机磷菌株143株。各菌株生长速度及形态特征差异较大。7种禾草根际溶解无机和有机磷菌株数量,除醉马草(Achnatherum inebrians)溶解无机磷呈根表土壤根系表面根内的分布趋势外,其他均呈现出根系表面根表土壤根内的分布趋势。大部分溶解无机磷菌株在10 d时溶磷圈直径(D)与菌落直径(d)的比值(D/d)不再增大,而溶解有机磷菌株则在14 d时不再增大。利用钼蓝比色法测定菌株溶磷能力,结果表明,109株溶解无机磷菌株培养液中有效磷含量在0.47~582.46μg·m L-1,最大为菌株PCRP5;143株溶解有机磷菌株培养液中有效磷含量在0.07~14.76μg·m L-1,最大为菌株MCMRS4;蒙金娜培养基pH与有机磷菌株有效磷含量无显著相关性(P0.05),PKO培养基pH与无机磷菌株有效磷含量之间呈极显著负相关(P0.01)。     

苜蓿根瘤菌溶磷和分泌植物生长素能力研究

对甘肃庆阳、天水、定西、武威和甘南5个不同生态区域栽培的陇东苜蓿和阿尔冈金苜蓿根瘤菌资源进行调查、分离和纯化,获得730多个分离物。分离物回接到原宿主植物检测其促生能力,筛选出29个较好的根瘤菌株,对其溶磷、分泌植物生长素能力进行了初步研究。结果表明,供试菌株都能够溶解有机磷(蛋黄卵磷脂EYPC),而不能溶解无机磷[Ca3(PO4)2];均能够分泌植物生长素(IAA),其中34.5%分泌能力较强,55.2%分泌能力中强,10.3%分泌能力较弱,分泌IAA的菌株数量比率高于禾本科根际分泌IAA菌株数量比率。     

长期饲喂高鸡肝饲粮所致犬尿结石成分的分析研究

对１例长期饲喂高鸡肝饲粮所致犬尿结石样品,利用化学定性、Ｘ－射线衍射、扫描电镜观察与能谱分析等手段进行化学组成和显微形态分析。结果表明,样品中棺盖状晶体、蜂窝状颗粒及某些形状的颗粒均以磷酸铵镁为主要组成成分,形状不甚清晰的小粒状颗粒以磷酸钙为主要成分。另外还对数份饲喂高鸡肝饲粮之犬尿沉渣进行光学显微镜观察,发现有大量磷酸盐及少量碳酸钙。作者对尿石的形成及尿沉渣与尿石的关系进行了简要讨论,并对宠物犬尿石症的预防提出了建议     

Development of zygotic and somatic embryos of Phoenix dactylifera L. cv. Deglet Nour: Comparative study

In order to improve somatic embryogenesis production in date palm Phoenix dactilyfera L. cv. Deglet Nour (DN), a comparative study between somatic (SE) and zygotic (ZE) embryos developments was carried out. The data showed that ZE maturation occurred from 10 to 19 weeks after pollination (WAP). During this period, the fresh weight (FW) and the dry weight (DW) of ZE increased progressively to reach a maximum level at 19 WAP. SE development occurred in three distinct stages. The DW remained constant during the two first stages, and declined slightly during the third and final stage. Embryo protein analysis revealed significant differences between ZE and SE. The ZE total protein level was initially low and increased to the maximum at mature stage. However, no significant change in total protein was detected during SE development. SDS-PAGE analysis showed a poor protein profile for SE, compared to that of ZE. In the latter, a 22 kDa protein was identified by N-terminal sequencing as a glutelin. This protein was accumulated rapidly during early development and remained at a relatively constant level during ZE development, and then declined progressively 12 days after embryo germination (DG). This protein seems to be absent in SE.